Date of Award
Master of Science (MS)
Using the Kitasato hypothesis as a basis, the effect of H+and light on Nitella was investigated. Upon a light to dark transition, the cell resting potential at pH 5.7 depolarized, whereas at pH 4.7 the membrane became hyperpolarized. Membrane conductance and H+ extrusion were not significantly affected by the pH change 5.7 to 4.7. Light promoted increases in both membrane conductance and H+ fluxes. All cells except those having extreme resting potential values, either high or low, were responsive to light. The action spectra for H+ flux, resting potential and membrane conductance is not the same as that for photosynthesis suggesting the possibility of another more complex mechanism(s) being involved with the light effects on Nitella.
Brown, Danley F., "The Effect of the Hydrogen Ion and Light on the Membrane Properties of Nitella" (1971). Environmental Science and Ecology Theses. 14.