Date of Award

10-21-2013

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Environmental Science and Biology

Abstract

The main objectives of this study were to determine and compare fatty acid signatures (FAS) of lake trout eggs within and among the Great Lakes region. Fifteen sites were sampled over 2 years, including six sites in Lake Michigan, four sites in Lake Huron and one site each in Lake Ontario, Lake Superior, Lake Champlain, and Cayuga Lake. A total of 518 egg samples were quantified. A combination of univariate and multivariate statistical analyses was used to assess spatial and temporal differences in FAS in both the neutral lipid (NL) and phospholipid (PL) fractions of lake trout eggs. At each sampling site, FAS did not differ significantly between the 2 years of sampling. Therefore samples from 2009 and 2010 were combined to assess spatial differences. Discriminant factor analysis (DFA) was performed on lake trout eggs from 13 sample sites using 18 of the most abundant fatty acids detected. DFA revealed a clear separation of lake trout eggs by sample site reaching an overall classification success of 77.7% and 77.3% in the neutral lipid and phospholipid fractions, respectively. Similarly, nonmetric multidimensional scaling and SIMPER analyses revealed differences in FAS among sample sites in both lipid fractions. These differences were driven by 16:1n-7 and 18:1n-9 in the NL and by 16:0 and docosahexaenoic acid in the PL. We suggest that the differences observed in FAS in lake trout eggs among sample sites are reflective of the lake trout feeding habit.

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