Program

Location

Edwards Hall Lobby

Document Type

Poster Presentation (1 hour)

Description

Kytococcus sedentarius is an opportunistic gram positive skin pathogen that is of commercial interest as the only known producer of the antibiotic monesin. The complete genome sequence of K. sedentarius is available and computer annotated, but manual genome annotation confirming individual genes as functionally encoding specific proteins has not been completed. The purpose of this study was to annotate genes potentially involved in the maltose/maltodextrin transporter system in K. sedentarius. This transporter system belongs to the ATP-binding cassette (ABC) superfamily of proteins that use energy from ATP hydrolysis to transport essential nutrients into the cell. The maltose/maltodextrin transporter is found only in prokaryotes and its four constitutive domains include two copies of the ABC protein encoded by the MalK gene and two transmembrane domain proteins encoded by the MalF and MalG genes. An additional cytoplasmic binding protein required for transporter function is encoded by the gene MalE. We used IMG-ACT to run a suite of software programs to analyze sequence information of candidate genes to determine their function. We successfully annotated MalE, MalF, and MalG genes within the K. sedentarius genome. However, the MalK encoding sequence had not been computer called and required additional analysis to identify candidate genes. Using the defined MalK gene sequences from the gram positive bacterium Alicyclobacillus acidocaldarius as a template, a homologous gene sequence that could potentially encode MalK protein in K. sedentarious was defined (Ksed_00570). Further studies are needed to confirm the function of the MalK gene product.

Start Date

20-4-2013 1:45 PM

Comments

Sciences & Engineering Poster Session

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Life Sciences Commons

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Apr 20th, 1:45 PM

Maltose/Maltodextrin ABC Transporter Structural Annotation in Kytococcus Sedentarius

Edwards Hall Lobby

Kytococcus sedentarius is an opportunistic gram positive skin pathogen that is of commercial interest as the only known producer of the antibiotic monesin. The complete genome sequence of K. sedentarius is available and computer annotated, but manual genome annotation confirming individual genes as functionally encoding specific proteins has not been completed. The purpose of this study was to annotate genes potentially involved in the maltose/maltodextrin transporter system in K. sedentarius. This transporter system belongs to the ATP-binding cassette (ABC) superfamily of proteins that use energy from ATP hydrolysis to transport essential nutrients into the cell. The maltose/maltodextrin transporter is found only in prokaryotes and its four constitutive domains include two copies of the ABC protein encoded by the MalK gene and two transmembrane domain proteins encoded by the MalF and MalG genes. An additional cytoplasmic binding protein required for transporter function is encoded by the gene MalE. We used IMG-ACT to run a suite of software programs to analyze sequence information of candidate genes to determine their function. We successfully annotated MalE, MalF, and MalG genes within the K. sedentarius genome. However, the MalK encoding sequence had not been computer called and required additional analysis to identify candidate genes. Using the defined MalK gene sequences from the gram positive bacterium Alicyclobacillus acidocaldarius as a template, a homologous gene sequence that could potentially encode MalK protein in K. sedentarious was defined (Ksed_00570). Further studies are needed to confirm the function of the MalK gene product.