Event Title

Non-additive cis-regulatory interactions underlie pair-rule regulation of Drosophila wingless gene at the blastoderm stage

Location

Edwards Hall Lobby

Document Type

Poster Presentation (1 hour)

Description

This poster seeks to investigate if non-additive interactions are general for segment polarity genes and if Runt is the key factor involved.

We have studied two putative pair-rule response elements for the segment polarity gene wingless: wg3911, located from -3.9kb to -1.1kb upstream of the wg transcription start site, and wg2946, located from +2.9kb to +4.6kb downstream of the tss, in intron 2 of the wg gene. We previously found that wg3911 is indeed a pair-rule response element at the blastoderm stage and, moreover, that wild-type expression of a wg3911-lacZ reporter matches endogenous wg expression but responds differently than endogenous wg to Prd and Odd manipulation. We now find that a wg2946-lacZ reporter also drives expression along the A-P axis, indicative of a role in mediating pair-rule regulation. Interestingly, expression of this reporter differs from the endogenous wg pattern in a manner suggesting that interactions between this enhancer and the wg promoter are also regulated.

Start Date

April 2014

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Apr 26th, 1:00 PM

Non-additive cis-regulatory interactions underlie pair-rule regulation of Drosophila wingless gene at the blastoderm stage

Edwards Hall Lobby

This poster seeks to investigate if non-additive interactions are general for segment polarity genes and if Runt is the key factor involved.

We have studied two putative pair-rule response elements for the segment polarity gene wingless: wg3911, located from -3.9kb to -1.1kb upstream of the wg transcription start site, and wg2946, located from +2.9kb to +4.6kb downstream of the tss, in intron 2 of the wg gene. We previously found that wg3911 is indeed a pair-rule response element at the blastoderm stage and, moreover, that wild-type expression of a wg3911-lacZ reporter matches endogenous wg expression but responds differently than endogenous wg to Prd and Odd manipulation. We now find that a wg2946-lacZ reporter also drives expression along the A-P axis, indicative of a role in mediating pair-rule regulation. Interestingly, expression of this reporter differs from the endogenous wg pattern in a manner suggesting that interactions between this enhancer and the wg promoter are also regulated.