Presenter Information

Aaron Matthews, SUNY OswegoFollow

Academic Field

Biological Sciences

Faculty Mentor Name

Peter D. Newell, PhD.

Presentation Type

Oral Presentation

Abstract

Bacteriophage are unique genetic elements that play vital roles in the evolution of their bacterial hosts. Bacteriophage are abundant in the guts of a wide range of animals, and studies suggest they contribute to the microbial population structure and stability. A bacteriophage dubbed 14g was isolated from wild Drosophila melanogaster flies. This study employed the gut microbiota of Drosophila melanogaster to examine the stability and infection dynamics of this phage. The latency time of infection and number of phage produced per infected cell were measured utilizing results from top agar assays. The susceptibility of 32 bacterial strains was measured by identifying either: i) plaque forming units or ii) zones of clearance after exposure to bacteriophage 14g. Our results concluded that bacteriophage 14g has the ability to infect and kill gut bacteria from the Acetobacteraceae family, namely: Gluconobacter cerinus and Gloconobacter frateurii. A Polymerase Chain Reaction assay was developed to test for the presence of bacteriophage 14g in putatively infected hosts. The genomes of the 32 bacterial strains tested were compared to identify bacterial genes associated with phage susceptibility. This revealed candidate genes involved in phage resistance, which will be the subject of future study. In summary, this study introduced and developed tools for studying a bacteriophage in the model gut microbiota community of the common fruit fly.

Keywords

Virology, Lytic, Bacteriophage, gut, microbiota, genomics, Drosophila, melanogaster

Start Date

10-4-2015 9:30 AM

End Date

10-4-2015 11:00 AM

Location

Holmes 214

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Apr 10th, 9:30 AM Apr 10th, 11:00 AM

Testing the Sensitivity of 32 Acetobacteraceae family bacteria to Bacteriophage 14g: Investigating Interactions among the Gut Microbiota of Drosophila melanogaster

Holmes 214

Bacteriophage are unique genetic elements that play vital roles in the evolution of their bacterial hosts. Bacteriophage are abundant in the guts of a wide range of animals, and studies suggest they contribute to the microbial population structure and stability. A bacteriophage dubbed 14g was isolated from wild Drosophila melanogaster flies. This study employed the gut microbiota of Drosophila melanogaster to examine the stability and infection dynamics of this phage. The latency time of infection and number of phage produced per infected cell were measured utilizing results from top agar assays. The susceptibility of 32 bacterial strains was measured by identifying either: i) plaque forming units or ii) zones of clearance after exposure to bacteriophage 14g. Our results concluded that bacteriophage 14g has the ability to infect and kill gut bacteria from the Acetobacteraceae family, namely: Gluconobacter cerinus and Gloconobacter frateurii. A Polymerase Chain Reaction assay was developed to test for the presence of bacteriophage 14g in putatively infected hosts. The genomes of the 32 bacterial strains tested were compared to identify bacterial genes associated with phage susceptibility. This revealed candidate genes involved in phage resistance, which will be the subject of future study. In summary, this study introduced and developed tools for studying a bacteriophage in the model gut microbiota community of the common fruit fly.