Academic Field

Chemistry, Chemical Engineering

Faculty Mentor Name

Dr. Karina Ckless

Presentation Type

Oral Presentation

Abstract

Erinolaoluwa Araoye1, Dejhy Pyram1, Usha D. Hemraz2, Rajesh Sunasee1, Karina Ckless1*

1 Chemistry Department, State University of New York at Plattsburgh, Plattsburgh NY

2 National Research Council of Canada, Montreal Canada

Crystalline cellulose nanocrystal (CNC) has emerged as a novel material for a wide variety of important applications such as nanofillers, nanocomposites, surface coatings, regenerative medicine and drug and DNA delivery. CNC has a fiber-like structure with sizes in the range of 200-300 nm long and 5-50 nm wide. Despite the great potential applicability of CNC and its derivatives very little is known about their potential immunogenicity. Fiber-like materials have been known for evoking an immune response in particular for activating the NLRP3-inflammasome/IL-1β pathway. In this study we evaluated the capacity of CNC and its cationic derivatives CNC-g-poly(AEM)-1, CNC-g-poly(AEM)-2, CNC-g-poly(AEMA)-1 and CNC-g-poly(AEMA)-2 to stimulate NLRP3-inflammasome/IL-1β axis and enhance mitochondrial ROS. Mouse macrophages (J774.A1) were stimulated for 24h with 25, 50 and 100 µg/mL of CNC and its cationic derivatives. IL-1b secretion was analyzed by ELISA, mitochondrial function by JC-1 staining, cytochrome c release, ATP content and total and mitochondrial ROS was assessed by DCF and MitoSox staining, respectively. Mitochondrial ROS and extracellular ATP was significantly increased in cells treated with CNC-g-poly(AEMA)-2, which correlates with the strongest effects on IL-1β secretion. Our data also suggest that the increases in mitochondrial ROS and ATP release induced by this compound may be associated with their capability to evoke immune response.

Keywords

cellulose nanocrystal, nanomaterial, inflammation, NLRP3 inflammasome, IL-1β, ROS, macrophages

Start Date

10-4-2015 4:15 PM

End Date

10-4-2015 5:30 PM

Location

Hartwell Hall 125

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Apr 10th, 4:15 PM Apr 10th, 5:30 PM

Cellulose nanocrystal (CNC) cationic derivatives induce NLRP3 inflammasome-dependent IL-1β secretion associated with mitochondrial ROS production

Hartwell Hall 125

Erinolaoluwa Araoye1, Dejhy Pyram1, Usha D. Hemraz2, Rajesh Sunasee1, Karina Ckless1*

1 Chemistry Department, State University of New York at Plattsburgh, Plattsburgh NY

2 National Research Council of Canada, Montreal Canada

Crystalline cellulose nanocrystal (CNC) has emerged as a novel material for a wide variety of important applications such as nanofillers, nanocomposites, surface coatings, regenerative medicine and drug and DNA delivery. CNC has a fiber-like structure with sizes in the range of 200-300 nm long and 5-50 nm wide. Despite the great potential applicability of CNC and its derivatives very little is known about their potential immunogenicity. Fiber-like materials have been known for evoking an immune response in particular for activating the NLRP3-inflammasome/IL-1β pathway. In this study we evaluated the capacity of CNC and its cationic derivatives CNC-g-poly(AEM)-1, CNC-g-poly(AEM)-2, CNC-g-poly(AEMA)-1 and CNC-g-poly(AEMA)-2 to stimulate NLRP3-inflammasome/IL-1β axis and enhance mitochondrial ROS. Mouse macrophages (J774.A1) were stimulated for 24h with 25, 50 and 100 µg/mL of CNC and its cationic derivatives. IL-1b secretion was analyzed by ELISA, mitochondrial function by JC-1 staining, cytochrome c release, ATP content and total and mitochondrial ROS was assessed by DCF and MitoSox staining, respectively. Mitochondrial ROS and extracellular ATP was significantly increased in cells treated with CNC-g-poly(AEMA)-2, which correlates with the strongest effects on IL-1β secretion. Our data also suggest that the increases in mitochondrial ROS and ATP release induced by this compound may be associated with their capability to evoke immune response.