Academic Field

Biological Sciences

Faculty Mentor Name

James R. Jacob, Ph.D.

Presentation Type

Poster Presentation

Abstract

Human UBIAD1 has been indicated in the biosynthesis of vitamin k2 within the mitochondria. A recent study involving Drosophila showed that UBIAD1/Heix mutants exhibited severe mitochondrial dysfunction, including decreased ATP production. Vitamin k2 (MK4) was shown to be an effective electron carrier and helped to recover normal ATP production. The objective of this experiment is to further explore the potential of vitamin k2 as an electron carrier in mitochondria in the hamster model. To investigate the relation between UBIAD1, MK4 and ATP production, transfection of the Chinese hamster ovary (CHO) cell line with a novel species specific UBIAD1 siRNA based off the human UBIAD1 target sequence will be employed. Increasing concentrations of MK4 will be administered to both cultures. ATP production will be measured in both control and transfected CHO cultures to elicit the effect of MK4 on ATP production.

Keywords

UBIAD1, Vitamin k2, ATP production, siRNA

Start Date

10-4-2015 11:15 AM

End Date

10-4-2015 12:00 PM

Location

SERC House of Fields

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Apr 10th, 11:15 AM Apr 10th, 12:00 PM

The Effect of Vitamin k2 on ATP Production in CHO Cells Transfected with siRNA for UBiAD1

SERC House of Fields

Human UBIAD1 has been indicated in the biosynthesis of vitamin k2 within the mitochondria. A recent study involving Drosophila showed that UBIAD1/Heix mutants exhibited severe mitochondrial dysfunction, including decreased ATP production. Vitamin k2 (MK4) was shown to be an effective electron carrier and helped to recover normal ATP production. The objective of this experiment is to further explore the potential of vitamin k2 as an electron carrier in mitochondria in the hamster model. To investigate the relation between UBIAD1, MK4 and ATP production, transfection of the Chinese hamster ovary (CHO) cell line with a novel species specific UBIAD1 siRNA based off the human UBIAD1 target sequence will be employed. Increasing concentrations of MK4 will be administered to both cultures. ATP production will be measured in both control and transfected CHO cultures to elicit the effect of MK4 on ATP production.