Academic Field

Biological Sciences

Faculty Mentor Name

McMaster-Schuyl, Lynda

Presentation Title

Inducing RNAi by feeding

Presentation Type

Poster Presentation

Abstract

RNA interference (RNAi) is a technique that deliberately introducing dsRNA sequences identical to the sequence of a gene, then the result of the silence of the gene can be observed. RNAi can be activated in C. elegans, a roundworm commonly studied by researchers, by feeding worms bacteria expressing dsRNA. In this project, two RNAi strain of E. coli, dqy-11 and bli-1, will be introduced to C. elegans. Each RNAi “feeding strain” of bacteria contains a plasmid with a piece of DNA containing sequence from the gene it is designed to silence. The dsRNA eaten by the worms will turn down the gene and the results can be examined by any changes of the phenotypes.

Keywords

RNAi, C. elegans, gene silence

Start Date

10-4-2015 2:00 PM

End Date

10-4-2015 2:45 PM

Location

SERC House of Fields

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Apr 10th, 2:00 PM Apr 10th, 2:45 PM

Inducing RNAi by feeding

SERC House of Fields

RNA interference (RNAi) is a technique that deliberately introducing dsRNA sequences identical to the sequence of a gene, then the result of the silence of the gene can be observed. RNAi can be activated in C. elegans, a roundworm commonly studied by researchers, by feeding worms bacteria expressing dsRNA. In this project, two RNAi strain of E. coli, dqy-11 and bli-1, will be introduced to C. elegans. Each RNAi “feeding strain” of bacteria contains a plasmid with a piece of DNA containing sequence from the gene it is designed to silence. The dsRNA eaten by the worms will turn down the gene and the results can be examined by any changes of the phenotypes.