Date of Award
Master of Science (MS)
Dr. Robert J. McLean
A quantitative method for the measurement of agglutination among Chlamydomonas moewusii gametes has been presented. Using this technique, it was demonstrated that gametes possess a higher Con A isoagglutinability than vegetative cells and the + mating type is more isoagglutinable by Con A in both the vegetative and gametic states than the - mating type. Pairing is inhibited by both colchicine and trypsin treatments of gametes but agglutination is not significantly inhibited. Data from preliminary experiments in which ferritin Con A was used as a marker was suggestive of the fact that colchicine may have prevented the distal clustering of Con A receptor sites on flagellar tips during mating interactions. The involvement of Con A receptor sites in mating interactions is implicated and the mechanism of tipping is discussed in terms of the colchicine and trypsin data presented.
Sedita, Nancy Jane, "A Quantitative Study of Agglutination in Chlamydomonas Moewusii" (1981). Biology Master’s Theses. 104.